TkA03G532480 | RubberDandelionOmics

Gene overview

TkA03G532480

Reference: TK1151 Chr: Chr03 Coordinates: 133924400-133927071 Strand: + Type: protein_coding

cDNA / CDS / Protein Sequences

Available sequence downloads CDS FASTA Protein FASTA cDNA FASTA

cDNA

2188 bp · TkA03G532480|TK1151|cDNA

TTCATATACGTAGTATGGTATTCCATGAATATTCTCCTATTTTAACCCTTCCGATTATCTTGATGTAGTGTATTGTATTATGTTGTTATCACGACCACTTTTAATCCTGAAATGGGCCTTCTTGATGATATCAATAACCACAATAGCAGACTCTAGTCCTCCCGAAGAACCAGTCAAATGTTCAACCAATCTCACAAACTGCACCATCACCAACTCCTACGGGACATTTCCCGATCGTACCATCTGCCATGCAGCCGAGG...

CDS

Download CDS FASTA

1440 bp · TkA03G532480|TK1151|CDS

ATGACGGACTCTATTAGCACAGAAAATCTCAACCGTATTATTAAAATTGACAATGAAAGCATGCTTATTACGGTAGAAAGCGGTGTGACTTTGAAGCAACTCATTAATGAAGCTGCTAAAAGCGGGTTGGTTTTGCCTTATACTCCTTATTGGTGGGGGTTAACGATAGGAGGGATGTTGGGTACGGGTGCGCATGGTAGCACTCTTTGGGGTGAAGGTAGTGCGATTCATGATTATGTGGTTCAAATGCGGATTGTCAC...

Protein

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479 aa · TkA03G532480|TK1151|PROTEIN

MTDSISTENLNRIIKIDNESMLITVESGVTLKQLINEAAKSGLVLPYTPYWWGLTIGGMLGTGAHGSTLWGEGSAIHDYVVQMRIVTPSGADEGYAKVRTLEENRESDHGDMNAAKVSLGVLGVISQVTLRMQPLFKRSITLVTQNDTDLSDQVATFGRQHEFADMIWYPSQKRVVYRIDDRVASNVSGNGLWDHPGFRTVPSLVVHILRDIEEGQEERGDVAGKCGVGKLTTNTLIKSAYGITNNGVLFTGYPVVGYQN...

Flanking Sequence Download

Sequence orientation and download details

Upstream/downstream are capped at 10000 bp per side. Negative-strand genes are reverse-complemented so the final FASTA follows transcriptional 5′→3′ orientation.

ID Mapping

Input ID	`TkA03G532480`
TK1151 ID	TkA03G532480
OH-1004 ID	LXL04_010518
Relationship / confidence	Rbh Sequence Supported / Probable
Protein identity (%)	98.900
Protein coverage (%)	TK1151 97.286 / OH-1004 77.667 Coverage and evidence Coverage is the aligned span of the best-scoring HSP divided by representative protein length. A homolog match does not establish functional validation.
Source version	TK1151 / GWHBCHF00000000

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SNP / Indel variants and carrier samplesCRA009020 · TK1151

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Basic Information and Functional Annotation

Gene ID TkA03G532480

Location Chr03:133924400-133927071 (+)

Description Similar to GULLO6: Probable L-gulonolactone oxidase 6

Arabidopsis / Rice Reference Genes and Papers

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Arabidopsis

Reference gene ID	Possible function	Papers / DOI
`At2g46760`	May be involved in the biosynthesis of ascorbic acid.	No verified direct reference in this release
`At2g46750`	Catalyzes the oxidation of L-gulono-1,4-lactone to ascorbic acid.	The contribution of Arabidopsis homologs of L-gulono-1,4-lactone oxidase to the biosynthesis of ascorbic acid.2010 · DOI: 10.1271/bbb.100157
`At1g32300`	May be involved in the biosynthesis of ascorbic acid.	No verified direct reference in this release
`At2g46740`	Catalyzes the oxidation of L-gulono-1,4-lactone to ascorbic acid.	No verified direct reference in this release
`At5g56490`	May be involved in the biosynthesis of ascorbic acid.	No verified direct reference in this release

Rice

No hit for this species in the current source table.

Functional annotation source

View annotation sources

Functional annotation source: OH1004 · LXL04_010518 · GCA_047496595.1_OSU_TarKok_2.0. These OH1004 annotations are retrieved through ID mapping; the Arabidopsis/rice table below uses protein alignments from the query reference.

GO annotations (OH1004 source)

GO:0019853 L-ascorbic acid biosynthetic process Biological Process

GO:0005576 extracellular region Cellular Component

GO:0009505 plant-type cell wall Cellular Component

GO:0009507 chloroplast Cellular Component

GO:0016020 membrane Cellular Component

GO:0003885 D-arabinono-1,4-lactone oxidase activity Molecular Function

GO:0016491 oxidoreductase activity Molecular Function

GO:0050105 L-gulonolactone oxidase activity Molecular Function

GO:0071949 FAD binding Molecular Function

KEGG gene cross-reference

ath:AT1G32300

ath:AT2G46740

ath:AT2G46750

ath:AT2G46760

ath:AT5G56490

These KEGG gene identifiers are linked through homologous proteins; they do not establish pathway function for this gene.

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Gene expression

View expression in different studies, tissues and treatments. Results are presented separately by study and measurement unit.

View expression for this gene

Data sources and gene correspondence

Expression data come from public transcriptome studies. Reference genomes, sample metadata and measurement units are shown with the results. Ambiguous cross-reference correspondences do not select an expression record automatically; missing values do not mean zero expression.

Subcellular Localization Top 5

Computational predictions or homology annotations below do not replace localization experiments.

Lysosome / Vacuole0.334

Rank 1 · TkA03G532480 · TK1151

Endoplasmic reticulum0.210

Rank 2 · TkA03G532480 · TK1151

Extracellular0.209

Rank 3 · TkA03G532480 · TK1151

Cytoplasm0.114

Rank 4 · TkA03G532480 · TK1151

Cell membrane0.113

Rank 5 · TkA03G532480 · TK1151

LocPro · Direct prediction · Computational prediction, not experimental localization

Open Subcellular Module

Predicted Functional Domains

Domain results are from Pfam-A/HMMER; positions are amino-acid coordinates on the protein sequence.

PF01565FAD_binding_45-90 aa

PF04030ALO128-186 aa

PF04030ALO291-429 aa

PF22906GULLO2-like_3rd441-465 aa

Gene ID	Domain ID	Domain Name	Start aa	End aa	E-value	Score
TkA03G532480	`PF01565`	FAD_binding_4	5	90	1.8e-15	57.6
TkA03G532480	`PF04030`	ALO	128	186	2.5e-17	64.1
TkA03G532480	`PF04030`	ALO	291	429	2.5e-17	64.1
TkA03G532480	`PF22906`	GULLO2-like_3rd	441	465	1.3e-11	44.6

Arabidopsis / Rice Protein Homology

Only Arabidopsis and rice are shown. Swiss-Prot entries are reviewed; TrEMBL entries are supplementary sequence candidates. Similarity is not experimental validation.

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Protein	Organism	Description	Identity	Database	Confidence
`O81032`	Arabidopsis thaliana	sp\|O81032\|GGLO6_ARATH Probable L-gulonolactone oxidase 6	62.500%	Swiss-Prot	High
`Q6NQ66`	Arabidopsis thaliana	sp\|Q6NQ66\|GGLO2_ARATH L-gulonolactone oxidase 2	61.000%	Swiss-Prot	High
`Q9C614`	Arabidopsis thaliana	sp\|Q9C614\|GGLO1_ARATH Probable L-gulonolactone oxidase 1	60.200%	Swiss-Prot	High
`O81030`	Arabidopsis thaliana	sp\|O81030\|GGLO5_ARATH L-gulonolactone oxidase 5	59.100%	Swiss-Prot	High
`Q9FM82`	Arabidopsis thaliana	sp\|Q9FM82\|GGLO4_ARATH Probable L-gulonolactone oxidase 4	59.500%	Swiss-Prot	High

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