TkA08G257340 | RubberDandelionOmics

Gene overview

TkA08G257340

Reference: TK1151 Chr: Chr08 Coordinates: 67097514-67102954 Strand: - Type: protein_coding

cDNA / CDS / Protein Sequences

Available sequence downloads CDS FASTA Protein FASTA cDNA FASTA

cDNA

2186 bp · TkA08G257340|TK1151|cDNA

TGACAAATACTTTCCGTCAAAATTCGACTTTCGCTATGTTTACCGGTCACCAAGCCCGTCGCCAGATCGCCCACTCTCCTTCTCGGCCATCGCCAATTTCACCGGGCAACACCACTGGTTCGTCGACACCTAAATTCGTTGCATATCCCCGGAGACTGACTTGTATGTTGGTAGATCGATTCGATATAACTAATAGCTGATTTGTCTTCCTCTCGATCATCTGAACTCTGAAGCCATGGAATCCAAACAAGGAACAAAAC...

CDS

Download CDS FASTA

1344 bp · TkA08G257340|TK1151|CDS

ATGGAATCCAAACAAGGAACAAAACACACAATCTTAATGGTATCCGACTTCTTCTACCCAAATTTCGGAGGTGTAGAGAACCACATATACCTCCTCTCCCAATGCCTAATCAAACTCGGCCACAAGGTCATAGTCATGACCCACGCCTACTCAAACCGCACCGGGGTCCGCTACATGACAAACGGGTTAAAAGTCTATTACGTCCCTTGGAAGCCCTTCCTAATGCAAAACACCCTCCCAACCTTCTTCCCAACTCTCCC...

Protein

Download Protein FASTA

447 aa · TkA08G257340|TK1151|PROTEIN

MESKQGTKHTILMVSDFFYPNFGGVENHIYLLSQCLIKLGHKVIVMTHAYSNRTGVRYMTNGLKVYYVPWKPFLMQNTLPTFFPTLPIIRTILIREKITLVHGHQAFSTLCHEALMHARTMNYKVVFTDHSLYGFADIGSIHMNKILQFTLADVTQAICVSHTSKENTVLRSGLPPEKVFVIPNAVDTTMFKPASERVVMDVIVIVVISRLVYRKGADLLVEVIPEICRLYPNVRFVIGGDGPKRVRLEEMREKHFLQDR...

Flanking Sequence Download

Sequence orientation and download details

Upstream/downstream are capped at 10000 bp per side. Negative-strand genes are reverse-complemented so the final FASTA follows transcriptional 5′→3′ orientation.

ID Mapping

Input ID	`TkA08G257340`
TK1151 ID	TkA08G257340
OH-1004 ID	LXL04_033448
Relationship / confidence	Rbh High Identity Coverage Synteny / Strict
Protein identity (%)	98.700
Protein coverage (%)	TK1151 98.881 / OH-1004 92.133 Coverage and evidence Coverage is the aligned span of the best-scoring HSP divided by representative protein length. A homolog match does not establish functional validation.
Source version	TK1151 / GWHBCHF00000000

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SNP / Indel variants and carrier samplesCRA009020 · TK1151

Expand to load variant records for this gene.

Basic Information and Functional Annotation

Gene ID TkA08G257340

Location Chr08:67097514-67102954 (-)

Description Similar to PIGA: Phosphatidylinositol N-acetylglucosaminyltransferase subunit A

Arabidopsis / Rice Reference Genes and Papers

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Arabidopsis

Reference gene ID	Possible function	Papers / DOI
`At3g45100`	Necessary for the synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis (Probable).	SETH1 and SETH2, two components of the glycosylphosphatidylinositol anchor biosynthetic pathway, are required for pollen germination and tube growth in Arabidopsis.2004 · DOI: 10.1105/tpc.014407

Rice

No hit for this species in the current source table.

Functional annotation source

View annotation sources

Functional annotation source: OH1004 · LXL04_033448 · GCA_047496595.1_OSU_TarKok_2.0. These OH1004 annotations are retrieved through ID mapping; the Arabidopsis/rice table below uses protein alignments from the query reference.

GO annotations (OH1004 source)

GO:0006506 GPI anchor biosynthetic process Biological Process

GO:0009846 pollen germination Biological Process

GO:0009860 pollen tube growth Biological Process

GO:1990830 cellular response to leukemia inhibitory factor Biological Process

GO:0000506 glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex Cellular Component

GO:0005634 nucleus Cellular Component

GO:0005789 endoplasmic reticulum membrane Cellular Component

GO:0016020 membrane Cellular Component

GO:0030867 rough endoplasmic reticulum membrane Cellular Component

GO:0008194 UDP-glycosyltransferase activity Molecular Function

GO:0017176 phosphatidylinositol N-acetylglucosaminyltransferase activity Molecular Function

KEGG gene cross-reference

ath:AT3G45100

hsa:5277

mmu:18700

spo:2540986

These KEGG gene identifiers are linked through homologous proteins; they do not establish pathway function for this gene.

Open Annotation Module

Gene expression

View expression in different studies, tissues and treatments. Results are presented separately by study and measurement unit.

View expression for this gene

Data sources and gene correspondence

Expression data come from public transcriptome studies. Reference genomes, sample metadata and measurement units are shown with the results. Ambiguous cross-reference correspondences do not select an expression record automatically; missing values do not mean zero expression.

Subcellular Localization Top 5

Computational predictions or homology annotations below do not replace localization experiments.

Endoplasmic reticulum0.511

Rank 1 · TkA08G257340 · TK1151

Mitochondrion0.227

Rank 2 · TkA08G257340 · TK1151

Cytoplasm0.150

Rank 3 · TkA08G257340 · TK1151

Nucleus0.114

Rank 4 · TkA08G257340 · TK1151

Cell membrane0.084

Rank 5 · TkA08G257340 · TK1151

LocPro · Direct prediction · Computational prediction, not experimental localization

Open Subcellular Module

Predicted Functional Domains

Domain results are from Pfam-A/HMMER; positions are amino-acid coordinates on the protein sequence.

PF20706GT4-conflict12-52 aa

PF13439Glyco_transf_422-189 aa

PF13579Glyco_trans_4_423-184 aa

PF08288PIGA48-137 aa

PF00534Glycos_transf_1198-341 aa

PF20706GT4-conflict201-317 aa

PF13692Glyco_trans_1_4204-340 aa

PF13524Glyco_trans_1_2230-338 aa

Gene ID	Domain ID	Domain Name	Start aa	End aa	E-value	Score
TkA08G257340	`PF20706`	GT4-conflict	12	52	0.035	13.8
TkA08G257340	`PF13439`	Glyco_transf_4	22	189	5.6e-29	102.0
TkA08G257340	`PF13579`	Glyco_trans_4_4	23	184	2.4e-11	45.0
TkA08G257340	`PF08288`	PIGA	48	137	2.6e-42	144.1
TkA08G257340	`PF00534`	Glycos_transf_1	198	341	1.3e-29	103.6
TkA08G257340	`PF20706`	GT4-conflict	201	317	0.035	13.8
TkA08G257340	`PF13692`	Glyco_trans_1_4	204	340	1.4e-25	91.0
TkA08G257340	`PF13524`	Glyco_trans_1_2	230	338	4e-07	30.8
TkA08G257340	`PF08288`	PIGA	294	308	2.6e-42	144.1
TkA08G257340	`PF13524`	Glyco_trans_1_2	364	394	4e-07	30.8

Arabidopsis / Rice Protein Homology

Only Arabidopsis and rice are shown. Swiss-Prot entries are reviewed; TrEMBL entries are supplementary sequence candidates. Similarity is not experimental validation.

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Protein	Organism	Description	Identity	Database	Confidence
`Q94BX4`	Arabidopsis thaliana	sp\|Q94BX4\|PIGA_ARATH Phosphatidylinositol N-acetylglucosaminyltransferase subunit A	82.300%	Swiss-Prot	High

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