TkA06G086540 | RubberDandelionOmics

Gene overview

TkA06G086540

Reference: TK1151 Chr: Chr06 Coordinates: 23133779-23142442 Strand: - Type: protein_coding

cDNA / CDS / Protein Sequences

Available sequence downloads CDS FASTA Protein FASTA cDNA FASTA

cDNA

486 bp · TkA06G086540|TK1151|cDNA

ATGGGGTATCATCATCTTCTTTCTTACCCGCACTCTTCGTTTCTTGTTGTTACCGTCTTTGTTTTTATCATGGTTCAATTTGTTTTGTTTTCTTCGATGGAATGGAGTTCGGGCTCTTTAAGTGACCTCGATGTGTACCAAAAACTTGTGGGAATTTTGTTTCAGACTGTAAACACAAGGCATACCGGAGAAACAATTGTTGACTTGTCCACAATCAATGCTTCCGTATTGGTCTTGTTTGTCGTGATGATGTATCTCCC...

CDS

Download CDS FASTA

486 bp · TkA06G086540|TK1151|CDS

ATGGGGTATCATCATCTTCTTTCTTACCCGCACTCTTCGTTTCTTGTTGTTACCGTCTTTGTTTTTATCATGGTTCAATTTGTTTTGTTTTCTTCGATGGAATGGAGTTCGGGCTCTTTAAGTGACCTCGATGTGTACCAAAAACTTGTGGGAATTTTGTTTCAGACTGTAAACACAAGGCATACCGGAGAAACAATTGTTGACTTGTCCACAATCAATGCTTCCGTATTGGTCTTGTTTGTCGTGATGATGTATCTCCC...

Protein

Download Protein FASTA

161 aa · TkA06G086540|TK1151|PROTEIN

MGYHHLLSYPHSSFLVVTVFVFIMVQFVLFSSMEWSSGSLSDLDVYQKLVGILFQTVNTRHTGETIVDLSTINASVLVLFVVMMYLPPYTSFLPVTEESYEEGSTGSSKVMDNLIFSQLSYLVIFIIIVCITERKHIVQDPLNFNVLNIVVEVIRYEKICE

Flanking Sequence Download

Sequence orientation and download details

Upstream/downstream are capped at 10000 bp per side. Negative-strand genes are reverse-complemented so the final FASTA follows transcriptional 5′→3′ orientation.

ID Mapping

Input ID	`TkA06G086540`
TK1151 ID	TkA06G086540
OH-1004 ID	LXL04_017921
Relationship / confidence	Non Reciprocal Best Hit / Complex relationship
Protein identity (%)	63.300
Protein coverage (%)	TK1151 94.410 / OH-1004 28.938 Coverage and evidence Coverage is the aligned span of the best-scoring HSP divided by representative protein length. A homolog match does not establish functional validation.
Source version	TK1151 / GWHBCHF00000000

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SNP / Indel variants and carrier samplesCRA009020 · TK1151

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Basic Information and Functional Annotation

Gene ID TkA06G086540

Location Chr06:23133779-23142442 (-)

Description Similar to HKT1: Sodium transporter HKT1

Arabidopsis / Rice Reference Genes and Papers

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Arabidopsis

Reference gene ID	Possible function	Papers / DOI
`At4g10310`	Sodium transporter protein, which plays a central role in plant tolerance to salt.	The Arabidopsis HKT1 gene homolog mediates inward Na(+) currents in xenopus laevis oocytes and Na(+) uptake in Saccharomyces cerevisiae.2000 · DOI: 10.1104/pp.122.4.1249 AtHKT1 is a salt tolerance determinant that controls Na(+) entry into plant roots.2001 · DOI: 10.1073/pnas.241501798 Glycine residues in potassium channel-like selectivity filters determine potassium selectivity in four-loop-per-subunit HKT transporters from plants.2002 · DOI: 10.1073/pnas.082123799 Altered shoot/root Na+ distribution and bifurcating salt sensitivity in Arabidopsis by genetic disruption of the Na+ transporter AtHKT1.2002 · DOI: 10.1016/s0014-5793(02)03488-9 Functional analysis of AtHKT1 in Arabidopsis shows that Na(+) recirculation by the phloem is crucial for salt tolerance.2003 · DOI: 10.1093/emboj/cdg207 AtHKT1 facilitates Na+ homeostasis and K+ nutrition in planta.2004 · DOI: 10.1104/pp.104.042234 Microarray-based rapid cloning of an ion accumulation deletion mutant in Arabidopsis thaliana.2004 · DOI: 10.1073/pnas.0404780101

Rice

Reference gene ID	Possible function	Papers / DOI
`UniProt: A2WNZ9`	Sodium transporter that under salt stress mediates sodium exclusion in the phloem to prevent sodium transfer to young leaf blades and reproductive tissues (By similarity).	No verified direct reference in this release
`Os01g0307500`	Sodium transporter that under salt stress mediates sodium exclusion in the phloem to prevent sodium transfer to young leaf blades and reproductive tissues.	OsHKT1;5 mediates Na+ exclusion in the vasculature to protect leaf blades and reproductive tissues from salt toxicity in rice.2017 · DOI: 10.1111/tpj.13595
`Os02g0175000`	Functions as a highly-selective sodium transporter.	Diversity in expression patterns and functional properties in the rice HKT transporter family.2009 · DOI: 10.1104/pp.109.138008 Identification of rice cornichon as a possible cargo receptor for the Golgi-localized sodium transporter OsHKT1;3.2015 · DOI: 10.1093/jxb/erv069
`Os04g0607600`	Probable cation transporter.	No verified direct reference in this release

Functional annotation source

View annotation sources

Functional annotation source: OH1004 · LXL04_017921 · GCA_047496595.1_OSU_TarKok_2.0. These OH1004 annotations are retrieved through ID mapping; the Arabidopsis/rice table below uses protein alignments from the query reference.

GO annotations (OH1004 source)

GO:0006813 potassium ion transport Biological Process

GO:0006814 sodium ion transport Biological Process

GO:0006970 response to osmotic stress Biological Process

GO:0009651 response to salt stress Biological Process

GO:0035725 sodium ion transmembrane transport Biological Process

GO:2000649 regulation of sodium ion transmembrane transporter activity Biological Process

GO:0000139 Golgi membrane Cellular Component

GO:0005739 mitochondrion Cellular Component

GO:0005789 endoplasmic reticulum membrane Cellular Component

GO:0005886 plasma membrane Cellular Component

GO:0008324 monoatomic cation transmembrane transporter activity Molecular Function

GO:0015079 potassium ion transmembrane transporter activity Molecular Function

KEGG gene cross-reference

ath:AT4G10310

dosa:Os01g0307500

dosa:Os02g0175000

osa:4328468

These KEGG gene identifiers are linked through homologous proteins; they do not establish pathway function for this gene.

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Gene expression

View expression in different studies, tissues and treatments. Results are presented separately by study and measurement unit.

View expression for this gene

Data sources and gene correspondence

Expression data come from public transcriptome studies. Reference genomes, sample metadata and measurement units are shown with the results. Ambiguous cross-reference correspondences do not select an expression record automatically; missing values do not mean zero expression.

Subcellular Localization Top 5

Computational predictions or homology annotations below do not replace localization experiments.

Cell membrane0.408

Rank 1 · TkA06G086540 · TK1151

Endoplasmic reticulum0.264

Rank 2 · TkA06G086540 · TK1151

Lysosome / Vacuole0.147

Rank 3 · TkA06G086540 · TK1151

Cytoplasm0.128

Rank 4 · TkA06G086540 · TK1151

Golgi apparatus0.082

Rank 5 · TkA06G086540 · TK1151

LocPro · Direct prediction · Computational prediction, not experimental localization

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Predicted Functional Domains

Domain results are from Pfam-A/HMMER; positions are amino-acid coordinates on the protein sequence.

PF02386TrkH12-86 aa

PF02386TrkH109-154 aa

Gene ID	Domain ID	Domain Name	Start aa	End aa	E-value	Score
TkA06G086540	`PF02386`	TrkH	12	86	3.6e-08	33.0
TkA06G086540	`PF02386`	TrkH	109	154	3.6e-08	33.0

Arabidopsis / Rice Protein Homology

Only Arabidopsis and rice are shown. Swiss-Prot entries are reviewed; TrEMBL entries are supplementary sequence candidates. Similarity is not experimental validation.

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Protein	Organism	Description	Identity	Database	Confidence
`Q84TI7`	Arabidopsis thaliana	sp\|Q84TI7\|HKT1_ARATH Sodium transporter HKT1	53.400%	Swiss-Prot	High
`A2WNZ9`	Oryza sativa subsp. indica	sp\|A2WNZ9\|HKT15_ORYSI Cation transporter HKT1;5	53.100%	Swiss-Prot	High
`Q0JNB6`	Oryza sativa subsp. japonica	sp\|Q0JNB6\|HKT15_ORYSJ Cation transporter HKT1;5	53.100%	Swiss-Prot	High
`Q6H501`	Oryza sativa subsp. japonica	sp\|Q6H501\|HKT13_ORYSJ Cation transporter HKT1;3	49.400%	Swiss-Prot	Medium
`Q7XPF7`	Oryza sativa subsp. japonica	sp\|Q7XPF7\|HKT14_ORYSJ Probable cation transporter HKT1;4	46.800%	Swiss-Prot	Medium

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