TkA04G444950 | RubberDandelionOmics

Gene overview

TkA04G444950

Reference: TK1151 Chr: Chr04 Coordinates: 112157799-112160728 Strand: - Type: protein_coding

cDNA / CDS / Protein Sequences

Available sequence downloads CDS FASTA Protein FASTA cDNA FASTA

cDNA

1552 bp · TkA04G444950|TK1151|cDNA

TCGATATGTCACAGTGTCTGCGTTCCAAGTGTATGCGTCTTCCCAATTTGTACATCACTTTTTATCCCCTATATATCTGCCCTAAAATCTCATGTTCATATATCCACATTTCCCTTATCCGGTTATCCCACGATTACTTTCTTCCCTAAATCTTCTAAACTTAAGTGGCGGTTTTCGGACAATATAGTCTCGATTCCTGCAATAGTAAGAGCAATTCAAACACACATTCTCAGAGAATTACTCACGAGACTTCGGATTCT...

CDS

Download CDS FASTA

1068 bp · TkA04G444950|TK1151|CDS

ATGGATACAGGAATTACAAGCAATCAGCAACCCGAAAAAGAAGAAAAAATGAGCAAAGCAATGAAGAAATCTCTGCTTGCTATGAACTGCATAATGCTCGGCGTCGGAAACTGCGGTGGTCCGTTAGTTCAACGACTCTACTTCGTTAAAGGAGGCAAAAGAGTCTGGACATCAAGCTGGCTACAAACCGCCGGCTGGCCATTTCTGATCATCCCTGTAATCCTTTCATTCCTATACAAAAAACGAAACGGAAAAGAAAC...

Protein

Download Protein FASTA

355 aa · TkA04G444950|TK1151|PROTEIN

MDTGITSNQQPEKEEKMSKAMKKSLLAMNCIMLGVGNCGGPLVQRLYFVKGGKRVWTSSWLQTAGWPFLIIPVILSFLYKKRNGKETQLFSLKPSLFLPCAVLGVLTGVDDYLAAYGVSRLPVSTSALIIATQLAFTAGFAFLLVKQKFTAFTINFIFLLSIGAVVLALHTSSDRPAHESSLRYYEGFFMTLGASALYGFILPSIELTMKKAKQTVTYSLVMEMQIVISFFATAVCTIGMLINHDFKAIPREGRNFDLGE...

Flanking Sequence Download

Sequence orientation and download details

Upstream/downstream are capped at 10000 bp per side. Negative-strand genes are reverse-complemented so the final FASTA follows transcriptional 5′→3′ orientation.

ID Mapping

Input ID	`TkA04G444950`
TK1151 ID	TkA04G444950
OH-1004 ID	LXL04_031136
Relationship / confidence	Non Reciprocal Best Hit / Complex relationship
Protein identity (%)	61.300
Protein coverage (%)	TK1151 96.901 / OH-1004 93.316 Coverage and evidence Coverage is the aligned span of the best-scoring HSP divided by representative protein length. A homolog match does not establish functional validation.
Source version	TK1151 / GWHBCHF00000000

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SNP / Indel variants and carrier samplesCRA009020 · TK1151

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Basic Information and Functional Annotation

Gene ID TkA04G444950

Location Chr04:112157799-112160728 (-)

Description Similar to PUP3: Purine permease 3

Arabidopsis / Rice Reference Genes and Papers

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Arabidopsis

Reference gene ID	Possible function	Papers / DOI
`At1g28220`	May be involved in transport of purine derivatives during pollen germination and tube elongation.	Transport of cytokinins mediated by purine transporters of the PUP family expressed in phloem, hydathodes, and pollen of Arabidopsis.2003 · DOI: 10.1046/j.1365-313x.2003.01700.x
`At1g28230`	Proton-coupled purine transporter mediating adenine and trans-zeatin uptake.	Transport of cytokinins mediated by purine transporters of the PUP family expressed in phloem, hydathodes, and pollen of Arabidopsis.2003 · DOI: 10.1046/j.1365-313x.2003.01700.x Recycling of pyridoxine (vitamin B6) by PUP1 in Arabidopsis.2013 · DOI: 10.1111/tpj.12195
`At2g33750`	Mediates adenine transport.	Transport of cytokinins mediated by purine transporters of the PUP family expressed in phloem, hydathodes, and pollen of Arabidopsis.2003 · DOI: 10.1046/j.1365-313x.2003.01700.x
`At1g30840`	Probable purine permease 4.	No verified direct reference in this release
`At1g19770`	Purine permease implicated in ATP-dependent cytokinin translocation that controls the spatiotemporal landscape of cytokinin signaling.	Plant development regulated by cytokinin sinks.2016 · DOI: 10.1126/science.aaf7254

Rice

No hit for this species in the current source table.

Functional annotation source

View annotation sources

Functional annotation source: OH1004 · LXL04_031136 · GCA_047496595.1_OSU_TarKok_2.0. These OH1004 annotations are retrieved through ID mapping; the Arabidopsis/rice table below uses protein alignments from the query reference.

GO annotations (OH1004 source)

GO:0006863 purine nucleobase transport Biological Process

GO:0009624 response to nematode Biological Process

GO:0010184 cytokinin transport Biological Process

GO:0005737 cytoplasm Cellular Component

GO:0005886 plasma membrane Cellular Component

GO:0009507 chloroplast Cellular Component

GO:0016020 membrane Cellular Component

GO:0005345 purine nucleobase transmembrane transporter activity Molecular Function

GO:0015211 purine nucleoside transmembrane transporter activity Molecular Function

GO:0022857 transmembrane transporter activity Molecular Function

KEGG gene cross-reference

ath:AT1G28220

ath:AT1G28230

ath:AT2G33750

ath:AT4G18210

ath:AT4G18220

These KEGG gene identifiers are linked through homologous proteins; they do not establish pathway function for this gene.

Open Annotation Module

Gene expression

View expression in different studies, tissues and treatments. Results are presented separately by study and measurement unit.

View expression for this gene

Data sources and gene correspondence

Expression data come from public transcriptome studies. Reference genomes, sample metadata and measurement units are shown with the results. Ambiguous cross-reference correspondences do not select an expression record automatically; missing values do not mean zero expression.

Subcellular Localization Top 5

Computational predictions or homology annotations below do not replace localization experiments.

Cell membrane0.574

Rank 1 · TkA04G444950 · TK1151

Endoplasmic reticulum0.160

Rank 2 · TkA04G444950 · TK1151

Lysosome / Vacuole0.160

Rank 3 · TkA04G444950 · TK1151

Golgi apparatus0.081

Rank 4 · TkA04G444950 · TK1151

Cytoplasm0.080

Rank 5 · TkA04G444950 · TK1151

LocPro · Direct prediction · Computational prediction, not experimental localization

Open Subcellular Module

Predicted Functional Domains

Domain results are from Pfam-A/HMMER; positions are amino-acid coordinates on the protein sequence.

PF16913PUNUT25-344 aa

PF00892EamA58-167 aa

PF08636Pkr1122-165 aa

PF05613Herpes_U15187-228 aa

PF00892EamA226-332 aa

PF08636Pkr1227-240 aa

PF08636Pkr1323-343 aa

Gene ID	Domain ID	Domain Name	Start aa	End aa	E-value	Score
TkA04G444950	`PF16913`	PUNUT	25	344	1.5e-112	376.8
TkA04G444950	`PF00892`	EamA	58	167	5.5e-07	30.7
TkA04G444950	`PF08636`	Pkr1	122	165	6.2	7.8
TkA04G444950	`PF05613`	Herpes_U15	187	228	0.082	14.4
TkA04G444950	`PF00892`	EamA	226	332	5.5e-07	30.7
TkA04G444950	`PF08636`	Pkr1	227	240	6.2	7.8
TkA04G444950	`PF08636`	Pkr1	323	343	6.2	7.8

Arabidopsis / Rice Protein Homology

Only Arabidopsis and rice are shown. Swiss-Prot entries are reviewed; TrEMBL entries are supplementary sequence candidates. Similarity is not experimental validation.

Download current result

Protein	Organism	Description	Identity	Database	Confidence
`Q9FZ95`	Arabidopsis thaliana	sp\|Q9FZ95\|PUP3_ARATH Purine permease 3	54.800%	Swiss-Prot	High
`Q9FZ96`	Arabidopsis thaliana	sp\|Q9FZ96\|PUP1_ARATH Purine permease 1	55.800%	Swiss-Prot	High
`Q94GB1`	Arabidopsis thaliana	sp\|Q94GB1\|PUP2_ARATH Purine permease 2	53.200%	Swiss-Prot	High
`Q9SY29`	Arabidopsis thaliana	sp\|Q9SY29\|PUP4_ARATH Probable purine permease 4	34.200%	Swiss-Prot	Medium
`Q9FXH5`	Arabidopsis thaliana	sp\|Q9FXH5\|PUP14_ARATH Purine permease 14	27.000%	Swiss-Prot	Low

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