TkA01G138720 | RubberDandelionOmics

Gene overview

TkA01G138720

Reference: TK1151 Chr: Chr01 Coordinates: 35214750-35215547 Strand: - Type: protein_coding

cDNA / CDS / Protein Sequences

Available sequence downloads CDS FASTA Protein FASTA cDNA FASTA

cDNA

705 bp · TkA01G138720|TK1151|cDNA

ATGAGCACCATGTTCAACGGGAAGATCATTGGACTGGTGAATGGGACCGCCGCCGGGTGGGGAAACATGGGCGGTGGTGCCACACAGCTTTTGATGCCGGTGCTGTTCGATATTATCAAGAAGTCCGGTGCAACGCCGTTCACTGCATGGAGGATCGCGTTCTTTATACCTGGATGGATGAATGTTATAATGGGGATATTGGTGTTAACTCTCGGCCAAGATTTACCAGACGGTAACCTTGGTTCTCTTCAAAAGAAGGG...

CDS

Download CDS FASTA

705 bp · TkA01G138720|TK1151|CDS

ATGAGCACCATGTTCAACGGGAAGATCATTGGACTGGTGAATGGGACCGCCGCCGGGTGGGGAAACATGGGCGGTGGTGCCACACAGCTTTTGATGCCGGTGCTGTTCGATATTATCAAGAAGTCCGGTGCAACGCCGTTCACTGCATGGAGGATCGCGTTCTTTATACCTGGATGGATGAATGTTATAATGGGGATATTGGTGTTAACTCTCGGCCAAGATTTACCAGACGGTAACCTTGGTTCTCTTCAAAAGAAGGG...

Protein

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234 aa · TkA01G138720|TK1151|PROTEIN

MSTMFNGKIIGLVNGTAAGWGNMGGGATQLLMPVLFDIIKKSGATPFTAWRIAFFIPGWMNVIMGILVLTLGQDLPDGNLGSLQKKGDVTTDKFGYAVTNYRTWIFVLLYGYSMGVELTIDNVIAEYFYDRFDLKLHLAGIVAASFGMANILARPFGGFTSDFMAKRFGMRGRLWNLWLLQTAGGVFCLGLGLVNSLPLAITFMILFSIGAQAAWVDIRNHPFLSRRSLGIISG

Flanking Sequence Download

Sequence orientation and download details

Upstream/downstream are capped at 10000 bp per side. Negative-strand genes are reverse-complemented so the final FASTA follows transcriptional 5′→3′ orientation.

ID Mapping

Input ID	`TkA01G138720`
TK1151 ID	TkA01G138720
OH-1004 ID	LXL04_004524
Relationship / confidence	Multi Source To Oh1004 / Complex relationship
Protein identity (%)	93.300
Protein coverage (%)	TK1151 100.000 / OH-1004 44.925 Coverage and evidence Coverage is the aligned span of the best-scoring HSP divided by representative protein length. A homolog match does not establish functional validation.
Source version	TK1151 / GWHBCHF00000000

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SNP / Indel variants and carrier samplesCRA009020 · TK1151

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Basic Information and Functional Annotation

Gene ID TkA01G138720

Location Chr01:35214750-35215547 (-)

Description Similar to NRT2.1: High-affinity nitrate transporter 2.1

Arabidopsis / Rice Reference Genes and Papers

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Arabidopsis

Reference gene ID	Possible function	Papers / DOI
`At5g60770`	Involved in high-affinity nitrate transport.	Disruption of the nitrate transporter genes AtNRT2.1 and AtNRT2.2 restricts growth at low external nitrate concentration.2004 · DOI: 10.1007/s00425-004-1266-x
`At1g08090`	Involved in nitrate transport, but does not seem to be able to mediate transport by its own.	Major alterations of the regulation of root NO(3)(-) uptake are associated with the mutation of Nrt2.1 and Nrt2.2 genes in Arabidopsis.2001 · DOI: 10.1104/pp.127.1.262 Disruption of the nitrate transporter genes AtNRT2.1 and AtNRT2.2 restricts growth at low external nitrate concentration.2004 · DOI: 10.1007/s00425-004-1266-x The putative high-affinity nitrate transporter NRT2.1 represses lateral root initiation in response to nutritional cues.2005 · DOI: 10.1073/pnas.0504219102 A central role for the nitrate transporter NRT2.1 in the integrated morphological and physiological responses of the root system to nitrogen limitation in Arabidopsis.2006 · DOI: 10.1104/pp.105.075721 Dissection of the AtNRT2.1:AtNRT2.2 inducible high-affinity nitrate transporter gene cluster.2007 · DOI: 10.1104/pp.106.091223 Nitrate signaling and the two component high affinity uptake system in Arabidopsis.2007 · DOI: 10.4161/psb.2.4.3870
`At1g08100`	Involved in high-affinity nitrate transport.	Major alterations of the regulation of root NO(3)(-) uptake are associated with the mutation of Nrt2.1 and Nrt2.2 genes in Arabidopsis.2001 · DOI: 10.1104/pp.127.1.262 Disruption of the nitrate transporter genes AtNRT2.1 and AtNRT2.2 restricts growth at low external nitrate concentration.2004 · DOI: 10.1007/s00425-004-1266-x Dissection of the AtNRT2.1:AtNRT2.2 inducible high-affinity nitrate transporter gene cluster.2007 · DOI: 10.1104/pp.106.091223

Rice

Reference gene ID	Possible function	Papers / DOI
`Os02g0112100`	Involved in nitrate transport, but does not seem to be able to mediate transport by its own.	Spatial expression and regulation of rice high-affinity nitrate transporters by nitrogen and carbon status.2011 · DOI: 10.1093/jxb/erq403 Rice OsNAR2.1 interacts with OsNRT2.1, OsNRT2.2 and OsNRT2.3a nitrate transporters to provide uptake over high and low concentration ranges.2011 · DOI: 10.1111/j.1365-3040.2011.02335.x
`Os02g0112600`	Involved in nitrate transport, but does not seem to be able to mediate transport by its own.	Spatial expression and regulation of rice high-affinity nitrate transporters by nitrogen and carbon status.2011 · DOI: 10.1093/jxb/erq403 Rice OsNAR2.1 interacts with OsNRT2.1, OsNRT2.2 and OsNRT2.3a nitrate transporters to provide uptake over high and low concentration ranges.2011 · DOI: 10.1111/j.1365-3040.2011.02335.x

Functional annotation source

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Functional annotation source: OH1004 · LXL04_004524 · GCA_047496595.1_OSU_TarKok_2.0. These OH1004 annotations are retrieved through ID mapping; the Arabidopsis/rice table below uses protein alignments from the query reference.

GO annotations (OH1004 source)

GO:0010167 response to nitrate Biological Process

GO:0015706 nitrate transmembrane transport Biological Process

GO:0042128 nitrate assimilation Biological Process

GO:0048527 lateral root development Biological Process

GO:0071249 cellular response to nitrate Biological Process

GO:0005886 plasma membrane Cellular Component

GO:0016020 membrane Cellular Component

GO:0015112 nitrate transmembrane transporter activity Molecular Function

KEGG gene cross-reference

ath:AT1G08090

ath:AT1G08100

ath:AT5G60770

dosa:Os02g0112100

dosa:Os02g0112600

These KEGG gene identifiers are linked through homologous proteins; they do not establish pathway function for this gene.

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Gene expression

View expression in different studies, tissues and treatments. Results are presented separately by study and measurement unit.

View expression for this gene

Data sources and gene correspondence

Expression data come from public transcriptome studies. Reference genomes, sample metadata and measurement units are shown with the results. Ambiguous cross-reference correspondences do not select an expression record automatically; missing values do not mean zero expression.

Subcellular Localization Top 5

Computational predictions or homology annotations below do not replace localization experiments.

Cell membrane0.238

Rank 1 · TkA01G138720 · TK1151

Lysosome / Vacuole0.229

Rank 2 · TkA01G138720 · TK1151

Endoplasmic reticulum0.221

Rank 3 · TkA01G138720 · TK1151

Cytoplasm0.157

Rank 4 · TkA01G138720 · TK1151

Golgi apparatus0.132

Rank 5 · TkA01G138720 · TK1151

LocPro · Direct prediction · Computational prediction, not experimental localization

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Predicted Functional Domains

Domain results are from Pfam-A/HMMER; positions are amino-acid coordinates on the protein sequence.

PF07690MFS_14-213 aa

PF00973Paramyxo_ncap106-131 aa

Gene ID	Domain ID	Domain Name	Start aa	End aa	E-value	Score
TkA01G138720	`PF07690`	MFS_1	4	213	1.6e-08	34.6
TkA01G138720	`PF00973`	Paramyxo_ncap	106	131	0.46	10.1

Arabidopsis / Rice Protein Homology

Only Arabidopsis and rice are shown. Swiss-Prot entries are reviewed; TrEMBL entries are supplementary sequence candidates. Similarity is not experimental validation.

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Protein	Organism	Description	Identity	Database	Confidence
`Q9FJH8`	Arabidopsis thaliana	sp\|Q9FJH8\|NRT24_ARATH High affinity nitrate transporter 2.4	73.200%	Swiss-Prot	High
`O82811`	Arabidopsis thaliana	sp\|O82811\|NRT21_ARATH High-affinity nitrate transporter 2.1	70.700%	Swiss-Prot	High
`Q9LMZ9`	Arabidopsis thaliana	sp\|Q9LMZ9\|NRT22_ARATH High-affinity nitrate transporter 2.2	70.300%	Swiss-Prot	High
`P0DKG9`	Oryza sativa subsp. japonica	sp\|P0DKG9\|NRT21_ORYSJ High-affinity nitrate transporter 2.1	73.200%	Swiss-Prot	High
`P0DKH0`	Oryza sativa subsp. japonica	sp\|P0DKH0\|NRT22_ORYSJ High-affinity nitrate transporter 2.2	73.200%	Swiss-Prot	High

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