GO / KEGG 统计富集
在明确的原生基因背景上计算过度代表分析；基因注释与统计结果分开显示。
示例来自当前注释中的12个萜类骨架通路候选基因，仅用于演示。TK1151和OH1004独立分析，不自动跨材料转移或合并基因。
默认背景为所选GO类别或KEGG通路中有当前注释的原生基因；自定义背景也与同一注释集合取交集。缺失注释不等于缺失功能，当前注释覆盖范围会限制分析。
输入与注释覆盖
TK1151 原生基因总数: 45224 · 注释版本: native_plant_ORA_20261013
逐基因GO与KEGG注释
每个原生基因一行；GO包含is_a/part_of上溯条目。先显示最多50个基因，下载包含全部基因。所有条目均为同源转移的计算注释。
| 原生基因ID | GO | KEGG pathways / KO | 供体蛋白 / Identity / Query coverage |
|---|---|---|---|
| TkA01G624900 | GO:0003824 catalytic activity; GO:0004419 hydroxymethylglutaryl-CoA lyase activity; GO:0005488 binding; GO:0005622 intracellular anatomical structure; GO:0005737 cytoplasm; GO:0005739 mitochondrion; GO:0005759 mitochondrial matrix; GO:0006520 amino acid metabolic process; GO:0006551 L-leucine metabolic process; GO:0006552 L-leucine catabolic process; GO:0006629 lipid metabolic process; GO:0008152 metabolic process; GO:0008610 lipid biosynthetic process; GO:0009056 catabolic process; GO:0009058 biosynthetic process; GO:0009063 amino acid catabolic process; GO:0009081 branched-chain amino acid metabolic process; GO:0009083 branched-chain amino acid catabolic process; GO:0009507 chloroplast; GO:0009536 plastid; GO:0009987 cellular process; GO:0016829 lyase activity; GO:0016830 carbon-carbon lyase activity; GO:0016833 oxo-acid-lyase activity; GO:0019752 carboxylic acid metabolic process; GO:0031974 membrane-enclosed lumen; GO:0036094 small molecule binding; GO:0043167 ion binding; GO:0043169 cation binding; GO:0043226 organelle; GO:0043227 membrane-bounded organelle; GO:0043229 intracellular organelle; GO:0043231 intracellular membrane-bounded organelle; GO:0043233 organelle lumen; GO:0043436 oxoacid metabolic process; GO:0044238 primary metabolic process; GO:0044281 small molecule metabolic process; GO:0044282 small molecule catabolic process; GO:0044283 small molecule biosynthetic process; GO:0046395 carboxylic acid catabolic process; GO:0046872 metal ion binding; GO:0046951 ketone body biosynthetic process; GO:0070013 intracellular organelle lumen; GO:0110165 cellular anatomical structure; GO:0170039 proteinogenic amino acid metabolic process; GO:0170040 proteinogenic amino acid catabolic process; GO:1901568 fatty acid derivative metabolic process; GO:1901570 fatty acid derivative biosynthetic process | ath00280 Valine, leucine and isoleucine degradation; ath00650 Butanoate metabolism; ath01100 Metabolic pathways; ath04146 Peroxisome K01640 | O81027 72.0 / 77.59 |
统计富集结果
单侧超几何检验；BP、MF、CC和KEGG分别进行Benjamini–Hochberg校正。背景命中数2–5,000的全部条目参与校正，包括前景零命中的条目；表中仅显示有命中的条目，不只显示显著条目。FDR≤0.05作为显示用显著性阈值。
| 类别 | N 有效背景 | n 有效前景 | 参与校正条目数 | FDR≤0.05 | 未纳入统计的前景基因 |
|---|---|---|---|---|---|
| biological_process | 11100 | 1 | 3880 | 0 | 0 |
背景与前景均按原生基因去重。不同类别的N/n可能不同，因为注释覆盖不同；FDR只在该类别的检验集合内解释。
有命中条目总数: 21 · 当前页 1/1 · 每页50条，下载不受分页限制
| ID / 名称 | 类别 | k/n | K/N | 期望命中 | 富集倍数 | P | FDR (BH) | 命中基因 |
|---|---|---|---|---|---|---|---|---|
| GO:0046951 ketone body biosynthetic process | biological_process | 1/1 | 3/11100 | 0.00027 | 3.7e+03 | 0.00027 | 1 | TkA01G624900 |
| GO:0006552 L-leucine catabolic process | biological_process | 1/1 | 11/11100 | 0.000991 | 1.01e+03 | 0.000991 | 1 | TkA01G624900 |
| GO:0006551 L-leucine metabolic process | biological_process | 1/1 | 16/11100 | 0.00144 | 694 | 0.00144 | 1 | TkA01G624900 |
| GO:0009083 branched-chain amino acid catabolic process | biological_process | 1/1 | 24/11100 | 0.00216 | 462 | 0.00216 | 1 | TkA01G624900 |
| GO:0009081 branched-chain amino acid metabolic process | biological_process | 1/1 | 37/11100 | 0.00333 | 300 | 0.00333 | 1 | TkA01G624900 |
| GO:0170040 proteinogenic amino acid catabolic process | biological_process | 1/1 | 50/11100 | 0.0045 | 222 | 0.0045 | 1 | TkA01G624900 |
| GO:1901570 fatty acid derivative biosynthetic process | biological_process | 1/1 | 53/11100 | 0.00477 | 209 | 0.00477 | 1 | TkA01G624900 |
| GO:0009063 amino acid catabolic process | biological_process | 1/1 | 57/11100 | 0.00514 | 195 | 0.00514 | 1 | TkA01G624900 |
| GO:1901568 fatty acid derivative metabolic process | biological_process | 1/1 | 66/11100 | 0.00595 | 168 | 0.00595 | 1 | TkA01G624900 |
| GO:0046395 carboxylic acid catabolic process | biological_process | 1/1 | 111/11100 | 0.01 | 100 | 0.01 | 1 | TkA01G624900 |
| GO:0170039 proteinogenic amino acid metabolic process | biological_process | 1/1 | 138/11100 | 0.0124 | 80.4 | 0.0124 | 1 | TkA01G624900 |
| GO:0044282 small molecule catabolic process | biological_process | 1/1 | 182/11100 | 0.0164 | 61 | 0.0164 | 1 | TkA01G624900 |
| GO:0006520 amino acid metabolic process | biological_process | 1/1 | 230/11100 | 0.0207 | 48.3 | 0.0207 | 1 | TkA01G624900 |
| GO:0044283 small molecule biosynthetic process | biological_process | 1/1 | 439/11100 | 0.0395 | 25.3 | 0.0395 | 1 | TkA01G624900 |
| GO:0008610 lipid biosynthetic process | biological_process | 1/1 | 462/11100 | 0.0416 | 24 | 0.0416 | 1 | TkA01G624900 |
| GO:0019752 carboxylic acid metabolic process | biological_process | 1/1 | 551/11100 | 0.0496 | 20.1 | 0.0496 | 1 | TkA01G624900 |
| GO:0043436 oxoacid metabolic process | biological_process | 1/1 | 600/11100 | 0.0541 | 18.5 | 0.0541 | 1 | TkA01G624900 |
| GO:0006629 lipid metabolic process | biological_process | 1/1 | 761/11100 | 0.0686 | 14.6 | 0.0686 | 1 | TkA01G624900 |
| GO:0044281 small molecule metabolic process | biological_process | 1/1 | 1043/11100 | 0.094 | 10.6 | 0.094 | 1 | TkA01G624900 |
| GO:0009056 catabolic process | biological_process | 1/1 | 1124/11100 | 0.101 | 9.88 | 0.101 | 1 | TkA01G624900 |
| GO:0009058 biosynthetic process | biological_process | 1/1 | 2662/11100 | 0.24 | 4.17 | 0.24 | 1 | TkA01G624900 |
富集图
按FDR排序显示前10个有命中条目，包含可能不显著的条目；气泡面积随命中数增加，图中标出k值。图示−log10 FDR上限为300，原始log P/log FDR保留在下载中。
手机上可横向滑动查看完整图，保留可读的文字大小。
富集倍数与命中数
校正后的显著性
方法、来源与解释范围
独立使用两套原生蛋白的首位Swiss-Prot拟南芥同源命中，阈值为Identity≥30%、Query coverage≥70%、E≤10⁻⁵。该注释集较保守，未达到阈值的其他基因仍保留在网站原始同源与注释模块中。GO剔除过时条目，规范化别名，并按固定的橡胶草物种约束筛查；仅沿is_a与part_of上溯。KEGG依据供体基因的KO与真实拟南芥通路映射，不把供体基因ID当作通路ID。
同源转移不等于已确认的直系同源或本物种实验功能。富集反映当前注释集合中的统计过度代表，不证明调控、通路激活或因果机制。转录组或蛋白组分析建议提交实验实际可检测的背景基因。
GO data-version: releases/2026-07-26 · 已排除的动物相关KEGG通路: ath04820, ath04620, ath04382, ath05100
查看源文件与SHA-256
ath_pathways.tsv · 2026-10-13T20:39:33+0800bdc8ed90c67e6f739fe1fef0742fff200d6dcbe264765a9070dff56bacf0b5ee
ath_gene_pathways.tsv · 2026-10-13T20:39:34+08006a2f07a143ecbc09b16c54ded5cb14e033cbe78eead30154c5bacb00ce9a5aea
ath_gene_ko.tsv · 2026-10-13T20:39:35+0800c408988440e9bed09f2d7ce3e6b4c7f0daf04167111344ba326263bc3f33c9d7
kegg_info.txt · 2026-10-13T20:39:36+080081e6c1b68c0afe91233190118bc0e94464b9f5c33114e3794e3b060b7975b0fb
ath_reviewed_go_kegg.tsv · 2026-10-13T20:48:07+0800684cb804a34ec241933dc67b2d1b9f0dc9a65332b92e8330f31ac28d927d2070
go-basic.obo · 2026-10-13T20:50:35+0800b08d45b268b8c24ccb2513dbbbc7d4df9f6521c099b413f79eb31e06e0fa3bcc
橡胶草物种约束文件SHA-256: 6e0c25c39c237c8e9223174f09420ac21f12b9f6ae5bccc9f431337f3da3fc5c